Also, it looks like your settings for first pass and second pass are almost too different. For example. Im pretty sure that Psy-rdo should be enabled on both passes.
Upping the bframes to 16 should help somewhat as well (since this is anime). an really, why aren't you using all partitions?
You can try the "Dup" plugin for avi-synth as well, it should offer some savings to you.
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