More reference frames usually help on animation but for normal content more then 5 is probably not very helpful. Generally from each reference frame you add you can expect about half as much quality gain then from the previous. In my opinion it's mostly the question how much time you are willing to spend on encoding and if you need hardware compatibility, in which case you have to choose an ammount appropriate for the resolution and level you encode to. Since you encode cartoons there are some things you should do, like lowering the aq strenght. Use search, it was discussed recently.
Also, drop "No Fast-P-Skip" and "No DCT-Decimate", they don't really help. Trellis 1 will also be much faster compared to the quality you lose.
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